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Background@#Dysfunction of vascular endothelial cells (ECs) plays a central role in the pathogenesis of cardiovascular complications in diabetes. SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 (SMARCA5) is a key regulator of chromatin structure and DNA repair, but its role in ECs remains surprisingly unexplored. The current study was designed to elucidate the regulated expression and function of SMARCA5 in diabetic ECs. @*Methods@#SMARCA5 expression was evaluated in ECs from diabetic mouse and human circulating CD34+ cells using quantitative reverse transcription polymerase chain reaction and Western blot. Effects of SMARCA5 manipulation on ECs function were evaluated using cell migration, in vitro tube formation and in vivo wound healing assays. Interaction among oxidative stress, SMARCA5 and transcriptional reprogramming was elucidated using luciferase reporter assay, electrophoretic mobility shift assay and chromatin immunoprecipitation. @*Results@#Endothelial SMARCA5 expression was significantly decreased in diabetic rodents and humans. Hyperglycemia-suppressed SMARCA5 impaired EC migration and tube formation in vitro, and blunted vasculogenesis in vivo. Contrarily, overexpression of SMARCA5 in situ by a SMARCA5 adenovirus-incorporated hydrogel effectively promoted the rate of wound healing in a dorsal skin punch injury model of diabetic mice. Mechanistically, hyperglycemia-elicited oxidative stress suppressed SMARCA5 transactivation in a signal transducer and activator of transcription 3 (STAT3)-dependent manner. Moreover, SMARCA5 maintained transcriptional homeostasis of several pro-angiogenic factors through both direct and indirect chromatin-remodeling mechanisms. In contrast, depletion of SMARCA5 disrupted transcriptional homeostasis to render ECs unresponsive to established angiogenic factors, which ultimately resulted in endothelial dysfunction in diabetes. @*Conclusion@#Suppression of endothelial SMARCA5 contributes to, at least in part, multiple aspects of endothelial dysfunction, which may thereby exacerbate cardiovascular complications in diabetes.
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We introduce the portfolio assessment into the classroom teaching reform in the curriculum of Cell Engineering, a specialty course in bioengineering & biotechnology. We established a complete classroom evaluation system that was divided the classroom assessment system of portfolio into four stages including the preparation stage, training stage, implementation stage and exhibition stage. We also discuss the feasibility and necessity of implementing the portfolio evaluation method in the course of cell engineering, the construction of evaluation system, and the key points and matters needing attention in the implementation process. The classroom reform is very productive, not only the classroom atmosphere has been activated, students' learning initiative and autonomy has been enhanced, but also the students' ability to analyze and solve professional problems related to cell engineering technology has been improved. The implementation of classroom teaching reform of this course can provide reference for other similar professional courses in colleges and universities.
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Humans , Cell Engineering , Curriculum , Learning , Students , UniversitiesABSTRACT
<p><b>OBJECTIVE</b>To evaluate the correlation of liver hardness testing</p><p><b>RESULTS</b>obtained by FibroTouch and FibroScan and the liver pathological stage.</p><p><b>METHODS</b>Seventy-five patients with chronic hepatitis B who presented to our clinic between January 2011 and April 2013 were examined with FibroTouch and FibroScan to evaluate the degree of liver fibrosis. Forty-six of those patients also underwent liver biopsy examination.</p><p><b>THE RESULTS</b>from technology-based testing and histopathological evaluation of the biopsy were compared by statistical analysis to determine the consistency of FibroTouch and FibroScan in regard to histological stage.</p><p><b>RESULTS</b>Analysis by paired t-test showed that the</p><p><b>RESULTS</b>from FibroTouch and FibroScan were not significantly different (t = -0.17, P =0.8616), and the correlation coefficient from Pearson's correlation analysis was 0.9949 (P less than 0.05), suggesting that the two technologies'</p><p><b>RESULTS</b>are correlated. Based on the histopathology</p><p><b>RESULTS</b>for liver fibrosis stage, the FibroTouch diagnosis of liver fibrosis more than or equal to S 1 had a receiver operating characteristic (ROC) area under the curve (AUC) of 0.889, diagnosis of liver fibrosis more than or equal to S2 had a ROC AUC of 0.941, diagnosis of liver fibrosis more than or equal to S3 had a ROC AUC of 0.908, and diagnosis of liver fibrosis more than or equal to S4 had a ROC AUC of 0.911.</p><p><b>CONCLUSION</b>Compared to FibroScan, FibroTouch has a better ability for detecting liver fibrosis and a better consistency with liver pathological stage determined by histopathological analysis.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Area Under Curve , Biopsy , Case-Control Studies , Elasticity Imaging Techniques , Hepatitis B, Chronic , Pathology , Liver , Pathology , Prospective StudiesABSTRACT
@#目的观察高压氧联合低频脉冲电刺激对脑梗死后假性球麻痹吞咽困难的康复治疗效果。方法脑梗死假性球麻痹吞咽困难患者120例，分为对照组、电刺激组及联合高压氧组，每组40例。所有患者接受脑卒中常规治疗和护理。观察吸入性肺炎、吞咽困难恢复情况。结果联合高压氧组吞咽困难康复总有效率为92.5%，高于对照组和电刺激组(P<0.05)；吸入性肺炎发生率为10.0%，低于对照组。结论高压氧联合低频脉冲电刺激对脑梗死后假性球麻痹具有治疗作用。
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Objective To make the validation of intravascular ultrasound elastography (IVUSE) for detecting the plaque.Methods A plain strain model of vascular plaque was established and then the RF signals of IVUS were produced.The radial displacement distribution and strain distribution were calculated through 1D numerical optimization based on the RF signals.Then the elastic moduli distribution was reconstructed by finite element method (FEM) and iteration method.Results The three elastic parameters,displacement/strain/moduli,all could detect the plaque.Elastic moduli presented the information of the shape and size of the plaque,meanwhile,the displacement and strain distributions could not give the information clearly.Conclusion Elastic modulus is proved to be superior to the other two parameters in detecting the plaque,whereas it is subject to the accuracy of the strain's estimation.